Our office is in the basement of Grays Hall in Harvard Yard. Our regular office hours are 10 AM to 5 PM from Monday to Friday. Feel free to drop by or make an appointment by either e-mailing harvfoun@fas.harvard.edu or calling at (617) 495-1527.
There are lots of ways to become involved with The Harvard Foundation! First, make sure to subscribe to our social media accounts so that you're aware of all our great events! If you're looking to be more involved in the creation and planning of events on campus you can volunteer as an associate or apply to be an intern.
Our hiring process typically begins early in the fall semester, when we release application forms here on the website. The application is composed of both a written and interview component. ...
Read this website thoroughly! Then do your research. Talk to faculty, tutors, and TFs from courses that you liked, and who have some expertise in your proposed field. Tell them what you are interested in, ask if they think it can be done in an existing program, and ask them who else to talk with. Talk to Directors of Undergraduate Studies in...
In the month of December, all applications and supporting materials that have been received are read and reviewed by a Committee of Faculty Reviewers, who then meet to discuss and decide on those students whom the Biophysics Program Co-Chairs and Program Administrator will invite to visit Harvard and the Biophysics Program as part of our annual Recruitment Visit/ Interview Weekend, The Biophysics Program confers with the Office of the Dean of the Graduate School of Arts and Sciences to receive approval for the proposed list of admitted students. The annual Recruitment Visit/...
Yes, WGS concentrators have spent semesters taking courses in countries such as Kenya, Australia, Spain, and France. With good planning, a semester abroad or out of residence can be a very meaningful educational experience.
ArcGIS Pro license is limited for use by valid Harvard ID holders only.
There are three licensing options (up to 6/30/2024):
Named user license (referred option): Login with your Harvard ID and HarvardKey to Harvard's ArcGIS Online Organization (harvard-cga.maps.arcgis.com) when you run your desktop ArcGIS Pro.
Concurrent use license: Run ArcGIS Pro from a computer that is on Harvard’s network (LAN or VPN), and use a concurrent use license from Harvard’s license server (license.cga.harvard.edu or cga2.cga....
The more pure your sample is, the easier it is to interpret the results, as contaminating proteins may contribute to your overall signal in bulk measurements.
However, if you wish to do conventional DSF (dye-based), please note that many hydrophobic dyes, such as Sypro Orange, cannot be used for Differential Scanning Fluorimetry of membrane proteins, dues to high background staining. However, if your membrane protein has buried cysteines, then a cysteine-reactive dye may work. BODIPY FL-cysteine can be used, since it has excitation and emission wavelengths within the standard range of many qPCR instruments, including our QuantStudio 6/7. CPM (a courmarin derivative) was one of the earlier dyes used for this purpose, however many modern qPCR instruments no longer have excitation filters appropriate for this dye.
MST experiments on the Monolith NT.115 pico require that one of the binding partners is fluorescently labeled.
For protein/protein interactions, start by considering the amounts, solubility and sequence of the proteins. The labeled target is typically used at 5-10 nM concentrations (200 ul/titration). The unlabeled ligand is ideally used at a concentration of 100X Kd (equilibrium dissociation constant), so could be uM to mM for weaker binders (20 ul/titration). If one protein is much more soluble and/or more abundant, that may be a better choice for the...
