Find the Goal challenges children to perform and interpreted mental states, perceptions, desires, beliefs, and intentional actions. Each round is played with a single deck of 16 cards, two plastic bins (one red, one blue) in which cards can be placed. The child will place the cards in the red or blue bin depending on their responses.
Mind Hunter challenges children to perform and interpret mental states, perceptions, desires, beliefs, and intentional actions. Each deck contains 16 cards, and your child must reason about what the card is depicting, and place the card on either a red or a blue piece of card based on which answer they think is correct (i.e. if the card shows a girl telling her friend that she likes swimming but not playing football, the friend will ask her to go swimming not to play football - if this is illustrated in the red box, the card should be placed in the red location...
Each round is played with a single deck of 16 cards and 2 large pieces of card (1 red, 1 blue) on which playing cards can be placed. The child will place the cards in the red or blue location depending on their responses.
The top of each two-sided card depicts a character who has a belief or a desire, which the child must reason about using either perceptual information or emotion, or interpreting symbols. For example, the character will be shown to desire a cake more than a cracker by symbolic information on one side (e.g. one thought bubble...
Number Chase is an untimed board game exercising counting, exact addition, and operations using the base-ten structure of the number system. It uses a game board, a deck of 16 cards, erasable colored markers, a scoring card, and an answer board. For the first deck, the game board presents 5 rows filled by the numbers 1-50, and the cards present simple addition problems presented as one array of black dots and one array of pink dots (Fig. 3a). In later decks, the pink dots will be replaced by a pink number.
1) Children find the box on the board whose number corresponds to the number of black dots (e.g., "3" on the board for 3 black dots).
2) They then move rightward on the board by one space for each dot in the array of pink dots (e.g., 4 rightward moves for the problem "3 black dots plus 4 pink dots").
3) Finally, they mark the space on the board with a marker.
The core has an Agilent Bravo NGS liquid handling platform used for library construction. The core also has an Agilent TapeStation 4200. Currently, core equipment is only available for use by core staff.
Invoices will be submitted to users by Harvard's Accounts Receivable department at the conclusion of library construction. Payment should be made to the address on the invoice you recieve from Accounts Receivable:
Harvard University Accounts Receivable 1033 Massachusetts Ave. 2nd Floor Cambridge, MA 02138
We accept Harvard 33-digit billing codes (required for internal users) and purchase orders. POs should be made out to the Harvard Accounts Receivable address above. For groups with multiple affiliations...
Currently the core offers two different nascent RNA library construction services: PRO-seq and TT-seq. Please see the links in the side-bar for detailed information about each of them and the table below for an overview of sample requirements.
PRO-seq (Precision Run-On Sequencing) offers single nucleotide resolution of nascent RNA 3' ends. This information can be used to identify candidate enhancer RNAs and profile the immediate transcriptional effects of experimental perturbations such as drug-treatment, cell differentiation, or protein degradation.
The method involves permeabilization of cells followed by a nuclear run-on assay in which engaged RNA polymerase complexes incorporate a single biotinylated nucleotide into the nascent RNA chain. These...
TT-seq is a variant of 4-thiouridine (4sU) sequencing. This approach labels newly synthesized RNA by metabolic incorporation of 4sU in live cells. Analysis of this RNA can provide information on RNA synthesis, co-transcriptional processing, and degradation (depending on experimental design).
Service Description
Users submit total RNA isolated from cells pulsed with 4sU. The core fragments the total RNA, enriches 4sU labeled fragments, constructs Illumina compatible RNA-seq libraries, and performs preliminary data...
