Lab Automation and Screening

Drug Discovery Sciences Core

Drug Discovery Sciences Core within the HMS Therapeutics Translator provides intellectual input, including chemistry advising and project management, as well as a variety of biochemical and cell-based assays in support of the therapeutics research initiatives of tenant companies within the Blavatnik Life Lab Longwood and the HMS community.

Contacts:
Jennifer Smith (Cellular & biochemical assays and structural biology expertise)
Mark Namchuk (Chemistry advising and project management expertise)
 

Drosophila RNAi Screening Center

The mission of the DRSC is to develop and optimize new molecular genetic technologies for use in vivo in Drosophila (TRiP technologies), as well as for use in Drosophila, mosquito, and other insect cell lines (DRSC technologies). In addition, we provide access to information, protocols, data and a suite of online tools via our website, and develop research resources such as new fly stocks and modified insect cell lines. We also have state-of-the-art facilities for in vivo Drosophila genetics and insect tissue culture, and provide support for small- and large-scale insect cell screening projects using RNAi or CRISPR-Cas technologies.

Core Director - Stephanie Mohr
Core Website - http://fgr.hms.harvard.edu/

HMS imaging cores website: https://microscopy.hms.harvard.edu

ICCB-Longwood Screening Facility

The ICCB-Longwood Screening Facility assists investigators in conducting high-throughput screens of chemical and functional genomics libraries to identify new tools for biological research. The ICCB-Longwood compound collection is continuously growing. Over 500,000 compounds are currently available for screening, including > ~15,000 'known bioactive' compounds, many of which have been characterized in animal models or in the clinic. Multiple human and mouse whole-genome siRNA libraries, as well as miRNA mimic and inhibitor libraries, are available for RNAi screening. Arrayed, synthetic single-guide RNA libraries targeting the human draggable genome are available for CRISPR knock out screening. Laboratory automation equipment is also available for use by the community for non-screening projects. The facility employs a staff-assisted screening model.

Core Director - Jennifer Smith
Core Assistant Director - Patricia Szajner
Core Website - http://iccb.med.harvard.edu/