A gene-centered C. elegans protein-DNA interaction network.

Citation:

Deplancke B, Mukhopadhyay A, Ao W, Elewa AM, a Grove C, Martinez NJ, Sequerra R, Doucette-Stamm L, Reece-Hoyes JS, a Hope I, et al. A gene-centered C. elegans protein-DNA interaction network. Cell. 2006;125(6):1193-205.

Abstract:

Transcription regulatory networks consist of physical and functional interactions between transcription factors (TFs) and their target genes. The systematic mapping of TF-target gene interactions has been pioneered in unicellular systems, using "TF-centered" methods (e.g., chromatin immunoprecipitation). However, metazoan systems are less amenable to such methods. Here, we used "gene-centered" high-throughput yeast one-hybrid (Y1H) assays to identify 283 interactions between 72 C. elegans digestive tract gene promoters and 117 proteins. The resulting protein-DNA interaction (PDI) network is highly connected and enriched for TFs that are expressed in the digestive tract. We provide functional annotations for approximately 10% of all worm TFs, many of which were previously uncharacterized, and find ten novel putative TFs, illustrating the power of a gene-centered approach. We provide additional in vivo evidence for multiple PDIs and illustrate how the PDI network provides insights into metazoan differential gene expression at a systems level.

Notes:

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