Gene silencing in Caenorhabditis elegans by transitive RNA interference.

Citation:

Alder MN, Dames S, Gaudet J, Mango SE. Gene silencing in Caenorhabditis elegans by transitive RNA interference. RNA (New York, N.Y.). 2003;9(1):25-32.

Abstract:

When a cell is exposed to double-stranded RNA (dsRNA), mRNA from the homologous gene is selectively degraded by a process called RNA interference (RNAi). Here, we provide evidence that dsRNA is amplified in Caenorhabditis elegans to ensure a robust RNAi response. Our data suggest a model in which mRNA targeted by RNAi functions as a template for 5' to 3' synthesis of new dsRNA (termed transitive RNAi). Strikingly, the effect is nonautonomous: dsRNA targeted to a gene expressed in one cell type can lead to transitive RNAi-mediated silencing of a second gene expressed in a distinct cell type. These data suggest dsRNA synthesized in vivo can mediate systemic RNAi.

Notes:

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