Publications

2015
Orth A, Tomaszewski MJ, Ghosh RN, Schonbrun E. Gigipixel multispectral microscopy. Optica. 2015.Abstract
Understanding the complexity of cellular biology often requires capturing and processing an enormous amount of data.  In high-content drug screens, each cell is labeled with several different fluorescent markers and frequently thousands to millions of cells need to be analyzed in order to characterize biology’s intrinsic variability.   In this work, we demonstrate a new microlens-based multispectral microscope designed to meet this throughput-intensive demand.  We report multispectral image cubes of up to 1.30 gigapixels in the spatial domain, with up to 13 spectral samples per pixel, for a total image size of 16.8 billion spatial-spectral samples.  To our knowledge, this is the largest multispectral microscopy dataset reported in the literature.  Our system has highly reconfigurable spectral sampling and bandwidth settings and we have demonstrated spectral unmixing of up to 6 fluorescent channels.
Caprio GD, Stokes C, Higgins JM, Schonbrun E. Single-cell measurement of red blood cell oxygen affinity. PNAS. 2015.Abstract
Oxygen is transported throughout the body by hemoglobin in red blood cells.  While the oxygen affinity of blood is well understood and is routinely assessed in patients by pulse oximetry, variability at the single-cell level has not been previously measured.  In contrast, single-cell measurements of red blood cell volume and hemoglobin concentration are taken millions of times per day by clinical hematology analyzers and are important factors in determining the health of the hematologic system.  To better understand the variability and determinants of oxygen affinity on a cellular level, we have developed a system that quantifies the oxygen saturation, cell volume and hemoglobin concentration for individual red blood cells in high-throughput.  We find that the variability in single-cell saturation peaks at an oxygen partial pressure of 2.5%, which corresponds to the maximum slope of the oxygen-hemoglobin dissociation curve.  In addition, single-cell oxygen affinity is positively correlated with hemoglobin concentration, but independent of osmolarity, which suggests variation in the hemoglobin to 2-3 DPG ratio on a cellular level.  By quantifying the functional behavior of a cellular population, our system adds a new dimension to blood cell analysis and other measurements of single-cell variability. 
2014
Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume
Schonbrun E, Malka R, Caprio GD, Schaak D, Higgins JM.

Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume

. Journal of Cytometry [Internet]. 2014. online
Polarization encoded color camera
Schonbrun E, Möller G, Caprio GD.

Polarization encoded color camera

. Optics Letters [Internet]. 2014;39(6):1433-1436. online
2013
Optical determination of intracellular water in apoptotic cells
Model MA, Schonbrun E.

Optical determination of intracellular water in apoptotic cells

. Journal of Physiology [Internet]. 2013;591(23):5843-5849. online
Hyperspectral fluorescence microfluidic microscopy
Caprio GD, Schaak D, Schonbrun E. Hyperspectral fluorescence microfluidic microscopy. Biomedical Optics Express [Internet]. 2013;4(8):1486-1493. Online
Dye exclusion cell volumetric imaging
Schonbrun E, Schaak D, Caprio GD. Dye exclusion cell volumetric imaging. Optics Express [Internet]. 2013;21(7):8793-8798. Online
Fluorescence imaging of flowing cells using a temporally coded excitation
Gorthi SS, Schaak D, Schonbrun E. Fluorescence imaging of flowing cells using a temporally coded excitation. Optics Express [Internet]. 2013;21(4):5164-517. Online
2012
Phase imaging flow cytometry using a focus-stack collecting microscope
Gorthi SS, Schonbrun E. Phase imaging flow cytometry using a focus-stack collecting microscope. Optics Letters. 2012;37(4):707-709.
Multiple field of view imaging flow cytometry
Schonbrun E, Gorthi SS, Schaak D. Multiple field of view imaging flow cytometry. Lab on a chip [Internet]. 2012;12:268-273. Abstract
2011
A microfluidic fluorescence measurement system using an astigmatic diffractive lens array
Schonbrun E, Steinvurzel P, Crozier KB. A microfluidic fluorescence measurement system using an astigmatic diffractive lens array. Optics Express [Internet]. 2011;19:1385. Online
Trapping and rotating nanoparticles using a plasmonic nano-tweezer with an integrated heat sink
Wang K, Schonbrun E, Steinvurzel P, Crozier KB. Trapping and rotating nanoparticles using a plasmonic nano-tweezer with an integrated heat sink. Nature Communications [Internet]. 2011;2:469. Abstract
Reconfigurable imaging systems using elliptical nanowires
Schonbrun E, Seo K, Crozier KB. Reconfigurable imaging systems using elliptical nanowires. Nano Letters [Internet]. 2011;11:4299-4303. Abstract
2010
Optical manipulation with planar silicon microring resonators
Lin S, Schonbrun E, Crozier KB. Optical manipulation with planar silicon microring resonators. Nano Letters [Internet]. 2010;10:2408-2411. Abstract
High throughput fluorescence detection using an integrated zone plate array
Schonbrun E, Abate A, Steinvurzel P, Weitz D, Crozier KB. High throughput fluorescence detection using an integrated zone plate array. Lab on a chip [Internet]. 2010;10:852. Abstract