Structure-inherent targeting of near-infrared fluorophores for parathyroid and thyroid gland imaging

Citation:

H. Hyun, M. H. Park, E. A. Owens, H. Wada, M. Henary, H. J. Handgraaf, A. L. Vahrmeijer, J. V. Frangioni, and H. S. Choi. 2015. “Structure-inherent targeting of near-infrared fluorophores for parathyroid and thyroid gland imaging.” Nat Med, 21, Pp. 192-7.

Abstract:

The typical method for creating targeted contrast agents requires covalent conjugation of separate targeting and fluorophore domains. In this study, we demonstrate that it is possible to create near-infrared (NIR) fluorophores with different tissue specificities driven by their inherent chemical structures. Thus, a single compact molecule performs both targeting and imaging. We use this strategy to solve a major problem in head and neck surgery: the identification and preservation of parathyroid and thyroid glands. We synthesized 700-nm and 800-nm halogenated fluorophores that show high uptake into these glands after a single intravenous (IV) injection of 0.06 mg kg(-1) in a pig. By using a dual-channel NIR imaging system, we observed-in real time and with high sensitivity-the unambiguous distinction of parathyroid and thyroid glands simultaneously in the context of blood and surrounding soft tissue. This novel technology lays a foundation for performing head and neck surgery with increased precision and efficiency along with potentially lower morbidity, and it provides a general strategy for developing targeted NIR fluorophores.

Notes:

Hyun, HoonPark, Min HoOwens, Eric AWada, HideyukiHenary, MagedHandgraaf, Henricus J MVahrmeijer, Alexander LFrangioni, John VChoi, Hak SooengR01-EB-010022/EB/NIBIB NIH HHS/R01-EB-011523/EB/NIBIB NIH HHS/R01-CA-115296/CA/NCI NIH HHS/R01 EB010022/EB/NIBIB NIH HHS/R01 EB011523/EB/NIBIB NIH HHS/Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tNat Med. 2015 Feb;21(2):192-7. doi: 10.1038/nm.3728. Epub 2015 Jan 5.