Gaudet Lab

Alumni

Trainees

Dates (Current position)

Project(s) and Other Notes

Graduate Students

Erik Procko (MCB)

2003-2009

(Assistant Professor U. of Illinois, Urbana-Champaign)

Thesis title: General Mechanisms for ABC Transporters revealed by the Transporter associated with Antigen Processing

Additional project: Role of TRPV1 ankyrin repeats in TRPV1 temperature sensitivity and calmodulin interactions

Ruiqi Rachel Wang

(Chem Bio)

2007-2009

(PhD 2012 with Tom Walz, Harvard Medical School; Assistant Director at Ntuitive Pt Ltd)

Biochemical characterization of the TRPV ankyrin repeats - the TRPV3 and TRPV4 ankyrin repeats bind ATP and calmodulin

Luciann L. Cuenca (BBS)

2009-2014

(Graduate Student, HMS)

Structural and functional studies of TRPV1 (NIH F31 Fellow)

Lukas Bane (MCO)

2011-2015 (D.E. Shaw Research)

Thesis title: Structural and Functional Studies into Nramp Divalent Metal Transporter Mechanisms

Monique Brewster (MCO)

2011-2016 (Preceptor, Harvard University)

Structural studies of the cytoplasmic domains of TRPA1 (NIH F31 Fellow)

Kenneth Skinner (Chemical Biology)

2012-2017 (Fellow, Vertex, San Diego, CA)

Propofol binding by the TRPA1 ion channel (NSF and Ford Fellow)

John Nicoludis

2012-2018 (Postdoctoral Fellow, UC San Francisco)

Structures of clustered protocadherins; Studies of MsrPQ

Aaron Bozzi

2012-2018 (Postdoctoral Fellow, MIT)

Structure and Function of Nramp transporters

Sri Srikant

2011-2019 (Postdoctoral Fellow, MIT)

Evolution of Substrate Selectivity in ATP Binding Cassette Exporters

Postdoctoral Fellows

Xiangshu Jin

2003-2006

(Assistant Investigator, NIBS, China)

Structure determination of the TRPV1 and TRPV2 ankyrin repeat domains

Christopher Phelps

2003-2009

(Principal Scientist, GlaxoSmithKline)

Functional and Structural Studies of TRPM8

Structure of the TRPV6 ankyrin repeats

Functional studies of TRPV channels

Wilhelm Weihofen

2005-2011

(Crystallography Group Leader, Novartis)

Structural studies of Nramp transporters

Structures of pathogen deubiquitinating enzymes (collaboration with H.L. Ploegh)

Structures of cadherins important in hearing (collaboration with D.P. Corey)

Sze Yi Lau

2008-2012

(Postdoc, A*STAR, Singapore)

Structural determinants of TRPV1 and calmodulin interaction

Functional studies of TRPM8

Marcos Sotomayor

2008-2013

(Assistant Professor, Ohio State University)

Structure and molecular dynamics of the hair cell tip link cadherins (collaboration with D.P. Corey) *HHW Fellow* *K99*

Ahmet Vakkasoglu

2009-2015 (Scientist, Momenta)

Structure and function of the TAP transporter

Alex Peralvarez-Marin

2010-2011

(Autonomous University of Barcelona, Spain)

Production of TRPV channels for structural studies *Marie Curie Fellow*

Ute Hellmich

2012-2014 (Assistant Professor, Johannes Gutenberg-Universität Mainz, Germany)

TRPV channel structural studies *EMBO Fellow*

Jeffrey McArthur

2013-2016

(Research Fellow, University of Wollongong, Australia)

Conformational changes in TRPV1

Christina Zimanyi

2013-2016 (Scientist, NY Structural Biology Center)

TRPA1 activation by electrophiles *Jane Coffin Childs Fellow*

Research Associates

Polina V. Lishko

2005-2007

(Assistant Professor, Berkeley)

Establishing Electrophysiology

Developing a cysteine-less TRPV1 protein

Role of the TRPV1 ankyrin repeats in TRPV1 function

Hitoshi Inada

2008-2012

(Lecturer, Tohoku University, Japan)

Expression of TRP channels in insect cells and yeast; evaluation using fluorescence size exclusion chromatography

Crystallization and structure determination of TRPV and TRPM channel domains

Undergraduates
Aaron Huang	2002-2004	Toward a TAP1/TAP2 nucleotide-binding domain heterodimer
Tiffany Hsieh	2002-2004 (MD, Columbia University)	Expression of the TRPV1 C-terminal domain
David Sola-Del Valle	2004-2005 (MD ’10, Columbia University, Ophthalmologist - Massachusetts Eye and Ear)	Thesis title: Structure-function studies of TRPA1, a thermo- and mechano-sensitive ion channel Hoopes Prize
Shelly S. Choo	2004-2006 (MD ’11, Johns Hopkins University)	Thesis title: Structural and Biochemical Studies of the N-Terminal Region of the Temperature-Sensing TRPV Ion Channels Hoopes and Henderson Prizes Co-author, JBC 2010; Nature Genetics 2010
Robert J. Huang	2005-2007 (MD, Duke University)	Thesis title: Structural and Functional Studies of the Ankyrin Repeats of TRPV Ion Channels Co-author, Biochemistry 2008
Samer Haidar	2005-2009 (MBA+MS in Mechanical Engineering, MIT)	Engineering Design Project title: Inducing Dimeric Interactions Using Leucine Zippers HHMI program
Albert Li	2006-2009 (MD ’13 Ophthalmology resident, NY)	Thesis title: Structural Studies of TRPV1 Activation by Capsaicin Hoopes Prize, PRISE Fellow
Vi Vu	2007-2009 (VES concentration, class of 2010)	Interaction of TRPV1 with calmodulin HHMI program, PRISE Fellow
Alejandra Beristain-Barajas	2007-2010 (Research technician)	Thesis title: Exploring the Molecular Mechanisms of Pain: Structural and Biochemical Studies on Nociceptor Channel TRPA1, Ankyrin Repeat Domain PRISE Fellow
Tianyi Zhu	Summer 2006	Expression and purification of bacterial Nramp transporters
Anne McCabe	2007-2011 (PhD student, Princeton)	Thesis title: Studying the ion permeation pathway of divalent metal transporter NRAMP using site-directed mutagenesis PRISE Fellow
Ahad Waraich	Summer 2008 (U. Chicago Class of 2012)	Biochemical characterization of the TRPM2 NUDIX domain and its interaction with nucleotides MCO/SROH Leadership Alliance Intern
Kevin C. Ni	2010-2012 (MD/PhD student, Indiana U)	Thesis title: Structural insights into asymmetric motor domains of the ABC transporter TAP PRISE Fellow
Robert Powers	2011-2014 (PhD student, Harvard Biophysics Program)	Thesis title: Structural study of a novel partial Ca2+-free linker and a positively selected variation in Protocadherin-15: Implications for hearing and cell adhesion PRISE Fellow, Hershel Smith Fellow, Hoopes Prize
Emily Lingeman	Summer 2012 (U. Wisconsin Madison ’14; PhD student at Berkeley)	Functional studies of NRAMP homologs MCO/SROH Leadership Alliance Intern
Alexandra Rojek	2012-2013 (Harvard ’15; MD student at UCSF)	Functional studies of NRAMP homologs
Erik Zheng	2012-2015 (MD/PhD student, Tri-Institute program, NY)	Thesis title: The molecular basis of capsaicin sensitivity in the nociceptor TRPV1 PRISE and Hershel Smith Fellow
Linda Honaker	Summer 2013 (SDSU ’14; PhD student, Harvard MCO program)	Structural studies of TRPV channels MCO/SROH Leadership Alliance Intern
Jenifer Brown	2013-2015 (PhD student at Stanford)	Thesis title: Engineered chimeric natural resistance associated macrophage proteins (NRAMPs) for antibody co-crystallization PRISE Fellow
Peter O’Byrne	Summer 2014 (Trinity College, Dublin Ireland ’15; PhD student, Trinity College)	Structural studies of clustered protocadherins
Patrick Bruck	Summer 2014 (Creighton University ’16; Research Assistant, Harvard Medical School)	Functional assays of yeast Ste6 transporter homologs MCO/SROH Leadership Alliance Intern
Hannah Zurier	2015-2017 (PhD student at Cornell University)	Role of the C-terminus in TRPA1 structure and function
Bennett Vogt	2015-2017 (Consultant, ClearView Life Sciences)	Assembly of clustered protocadherins
Benjamin Barnett	2015-2017	Structure and function of Nramp transporters
José Velilla	Summer 2015 (University of Florida ’16; PhD student, Harvard Chemical Biology)	Assembly of TRPC channel intracellular domains
Brandon Lee	2015-2016	Structure and function of Nramp transporters
Valia Leifer	Spring 2016	Membrane protein structure analyses
Stephanie Kabeche	Summer 2016 (University of Miami ’16; PhD Student at Stanford)	Structure of clustered protocadherins
Keri Ngo	Summer 2017 MCO SROH Intern	Structure of clustered protocadherins
Brenda Chiang	2018-2019	Interaction specificity of clustered protocadherins
Agnele Sewa	2018-2020 (Brandeis ’20)	Structural determinants of metal binding in Nramp transition metal transporters
Arianna Broad	Summer 2019 (Florida State University ’20)	Functional studies of insect gustatory receptors
Femke Ahlers	Summer 2019 (Cambridge University ’20)	Structural studies of Nramp transporters

Research Assistants
Jason Touhey	2004-2006 (Hospitalist)	Structure of the TRPV2 ankyrin repeats Co-author JBC 2006
Amanda McFedries	2008-2009 (Harvard PhD ’14; WarpDrive Bio Inc.)	Structure and function of the TAP transporter
Gavin MacKinnon	Extension School Volunteer 2008-2009 (MD Student, U. Michigan)	Cysteine-scanning mutagenesis of TRPV1

High school students

Serena Blacklow

2011-2013 (Harvard College ’17; MD/PhD Student, UCSF)

Lipid binding of TRPV ankyrin repeats

Julia Burnett

2015-2016 (Amherst College ’20)

TAP nucleotide binding domain dimerization

Research Interns

Nina Christ

Spring 2014 (Goethe University PhD ’14; Postdoc University of Mainz)

Structure and function of TRPV2 and TRPV4

Antonia Lott

2016-2017 (Eberhard Karls University)

Structural studies of bacterial Nramp homologs

Gaudet Lab

Members

Serin Baek

Sam Berry

Nhu Dang

Heather Frank

Rachelle Gaudet

Michael Gutierrez

Edward Lee

Jacob Licht

Elizabeth May

Mit Patel

Shamayeeta Ray

José Velilla

Unice Yoo

Gerardo Zavala

Casey Zhang

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Alumni

Trainees

Dates (Current position)

Project(s) and Other Notes