Publications

Nramp-Family Transition Metal Transporters

A.T. Bozzi, C.M. Zimanyi, J.M. Nicoludis, B.K. Lee, C.H. Zhang, R. Gaudet (2019) Structures in multiple conformations reveal distinct transition metal and proton pathways in an Nramp transporter. eLife 2019;8:e41124. PubMed: 30714568 PDB: 6C31 6BU5 6D91 6D9W

A.T. Bozzi, L.B. Bane, W.A. Weihofen, A. Singharoy, E.R. Guillen, H.L. Ploegh, K. Schulten, and R. Gaudet. (2016) Crystal Structure and Conformational Change Mechanism of a Bacterial Nramp-Family Divalent Metal Transporter. Structure 24, 2102-2114. PubMed: 27839948 PMCID: PMC5143219 PDB: 5KTE

A. T. Bozzi, L. B. Bane, W. A. Weihofen, A. L. McCabe, A. Singharoy, C. J. Chipot, K. Schulten, and R. Gaudet (2016) Conserved methionine dictates substrate preference in Nramp-family divalent metal transporters. Proc. Natl Acad. Sci. USA 113, 10310-5. PubMed: 27573840 PMCID: PMC5027461.

 

Structural Studies of TRP Channels

R.K. Finol-Urdaneta, J.R. McArthur, M.P. Goldschen-Ohm, R. Gaudet, D. B. Tikhonov, B.S. Zhorov, R.J. French. Batrachotoxin acts as a stent to hold open homotetrameric prokaryotic voltage-gated sodium channels. Journal of General Physiology (2018). PubMed: 30587506

B. Goretzki,  N.A. Glogowski, E. Diehl, E. Duchardt-Ferner, C. Hacker, R. Gaudet,  U.A. Hellmich. Structural Basis of TRPV4 N Terminus Interaction with Syndapin/PACSIN1-3 and PIP2. Structure (2018). PubMed: 30244966

K. Woll, K. Skinner, E. Gianti, N. Bhanu, B. Garcia, V. Carnevale, R. Eckenhoff, and R. Gaudet. Sites Contributing to TRPA1 Activation by the Anesthetic Propofol Identified by Photoaffinity Labeling. Biophysical Journal (2017 - In press). PubMed: 28935134 PMCID: PMC5700380

M.S.J. Brewster and R. Gaudet (2015) How the TRPA1 receptor transmits painful stimuli: Inner workings revealed by electron cryomicroscopy. BioEssays 37, 1184-1192. PubMed: 26387779 PMCID: PMC4862669

 

Z.A. Knecht, R. Gaudet, and P.A. Garrity (2015) The Touching Tail of a Mechanotransduction Channel. Cell 162, 1214-6. PubMed: 26359983 

 

R. Gaudet, B. Roux, and D. L. Minor Jr. (2015) Insights into the molecular foundations of electrical excitation. J Mol Biol. 427, 1-2. PubMed: 25542854 PMCID: PMC5544904

 

U.A. Hellmich, and R. Gaudet (2014) High-resolution views of TRPV1 and their implications for the TRP channel superfamily. Handb Exp Pharmacol. 223, 991-1004. PubMed: 24961977 PMCID: PMC5075239

 

U.A. Hellmich, and R. Gaudet (2014) Structural Biology of TRP Channels. Handb Exp Pharmacol. 223, 963-90. PubMed: 24961976 PMCID: PMC5075240

 

J. M. Sullivan, C. M. Zimanyi, W. Aisenberg, B. Bears, D.-H. Chen, J. W. Day, T. D. Bird, C. E. Siskind, R. Gaudet, and C. J. Sumner (2015) Novel mutations highlight the key role of the ankyrin repeat domain in TRPV4-mediated neuropathy. Neurology Genetics, 1, e29. PubMed: 27066566 PMCID: PMC4811381

 

J. M. Sullivan, G. Landouré, R. Gaudet, C. Sumner. WriteClick: Phenotypic spectrum and incidence of TRPV4 mutations in patients with Inherited axonal neuropathy. Neurology (2014) 83, 1991. PubMed: 25404646

A. Garcia-Elias, S. Mrkonjic, C. Pardo-Pastor, H. Inada, U. A. Hellmich, F. Rubio-Moscardó, C. Plata, R. Gaudet, R. Vicente, M. A. Valverde. Phosphatidylinositol-4,5-biphosphate-dependent rearrangement of TRPV4 cytosolic tails enables channel activation by physiological stimuli. Proc. Natl Acad. Sci. USA (2013) 110, 9553-9558. PubMed: 23690576 PMCID: PMC3677448.

A. Perálvarez-Marín, P. Doñate-Macian, and R. Gaudet (2013) What do we know about the transient receptor potential vanilloid 2 (TRPV2) ion channel? FEBS J. 280, 5471-87. PubMed: 23615321 PMCID: PMC3783526

S.Y. Lau, E. Procko, and R. Gaudet (2012) Distinct properties of Ca2+–calmodulin binding to N- and C-terminal regulatory regions of the TRPV1 channel. Journal of General Physiology 140, 541-555. PubMed: 23109716 PMCID: PMC3483115 PDB: 3SUI

H. Inada, E. Procko, M. Sotomayor and R. Gaudet. Structural and Biochemical Consequences of Disease-Causing Mutations in the Ankyrin Repeat Domain of the Human TRPV4 Channel. Biochemistry (2012) 51: 6195-206. PubMed: 22702953 PMCID: PMC3413242 PDB: 4DX1 4DX2

G. Landouré, J. M. Sullivan, J. O. Johnson, C. H. Munns, Y. Shi, O. Diallo, J. R. Gibbs, R. Gaudet, C. L Ludlow, K. H. Fischbeck, B. J. Traynor, B. G. Burnett and C. J. Sumner. Exome sequencing identifies a novel TRPV4 mutation in a CMT2C family. Neurology (2012) 79: 192-4. PubMed: 22675077 PMCID: PMC3390542

M. Zimon, J. Baets, M. Auer-Grumbach, J. Berciano, A. Garcia, E. Lopez-Laso, L. Merlini, D. Hilton-Jones, M. McEntagart, A. H. Crosby, N. Barisic, E. Boltshauser, C. E. Shaw, G. Landouré, C. L. Ludlow, R. Gaudet, H. Houlden, M. M. Reilly, K. H. Fischbeck, C. J. Sumner, V. Timmerman, A. Jordanova and P. De Jonghe. Dominant mutations in the cation channel gene transient receptor potential vanilloid 4 cause an unusual spectrum of neuropathies. Brain (2010) 133: 1798-809. PubMed: 20460441 PMCID: PMC2912694

G. Landouré, A.A. Zdebik, T.L. Martinez, B.G. Burnett, H.C. Stanescu, H. Inada, Y. Shi, A.A Taye, L. Kong, C.H Munns, S.S. Choo, C.B. Phelps, R. Paudel, H. Houlden, C.L. Ludlow1, M.J. Caterina, R. Gaudet, R. Kleta, K.H. Fischbeck and C.J. Sumner. Mutations in TRPV4 cause Charcot-Marie-Tooth disease type 2C. Nature Genetics (2010) 42: 170-4. PubMed: 20037586 PMCID: PMC2812627 PDB: 3JXI 3JXJ

C.B. Phelps, R.R. Wang, S.S. Choo and R. Gaudet. Differential regulation of TRPV1, TRPV3 AND TRPV4 sensitivity through a conserved binding site on the ankyrin repeat domain. J. Biol. Chem. (2010) 285: 731-40. PubMed: 19864432 PMCID: PMC2804222

R. Gaudet. Divide and conquer: high resolution structural information on TRP channel fragments. J Gen Physiol. (2009) 133: 231-7. PubMed:19237587 PMCID: PMC2654082

R. Gaudet. A primer on ankyrin repeat function in TRP channels and beyond. Mol Biosyst. (2008) 4: 372-9. Epub 2008 Mar 26. Review. PubMed:18414734 PMCID: PMC3006086

R. Gaudet. TRP channels entering the structural era. J Physiol. (2008) 586: 3565-75. Epub 2008 Jun 5. Review. PubMed:18535090 PMCID: PMC2538826

C.B. Phelps, R.J. Huang, P.V. Lishko, R.R. Wang and R. Gaudet. Structural analyses of the Ankyrin Repeat Domain of TRPV6 and related TRPV ion channels. Biochemistry (2008) 47: 2476-2484. PubMed 2RFA

C.B. Phelps and R. Gaudet The Role of the N-Terminus and Transmembrane Domain of TRPM8 in Channel Localization and Tetramerization.
Journal of Biological Chemistry (2007) 282: 36474-80.
PubMed: 17908685

C.B. Phelps, E. Procko, P.V. Lishko, R.R. Wang and R. Gaudet Insights into the roles of conserved and divergent residues in the ankyrin repeats of TRPV ion channels. Channels (2007) 1: 148-51. Epub 2007 Jul 9. PubMed: 18690026

P.V. Lishko, E. Procko, X. Jin, C.B. Phelps and R. Gaudet The ankyrin repeats of TRPV1 bind multiple ligands and modulate channel sensitivity.
Neuron (2007) 54: 905-918.
PubMed: 17582331 PDB: 2PNN 2NYJ

R. Gaudet Structural Insights into the Function of TRP Channels TRP Ion Channel Function in Sensory Transduction and Cellular Signaling Cascades, W. Liedtke, and S. Heller, eds. (Boca Raton, FL: CRC Press 2007), pp. 349-359. PubMed: 21204513

X. Jin, J. Touhey and R. Gaudet Structure of the N-terminal ankyrin repeat domain of the TRPV2 ion channel. J. Biol. Chem. (2006) 281: 25006-10. PubMed: 16809337 PDB: 2ETA 2ETB 2ETC

 

Structural Studies of TAP & Antigen Presentation

A.S. Vakkasoglu, S. Srikant, and R. Gaudet (2017) D-helix influences dimerization of the ATP-binding cassette (ABC) transporter associated with antigen processing 1 (TAP1) nucleotide-binding domain. PLoS One 12:e0178238. PubMed: 28542489 PMCID: PMC5441636.

N. Grossmann, A. S. Vakkasoglu, S. Hulpke, R. Abele, R. Gaudet*, R. Tampé* Mechanistic determinants of the directionality and energetics of active export by a heterodimeric ABC transporter. Nature Communications (2014) 5, 5419. PubMed: 25377891 PMCID: PMC4242082

E. Procko and R. Gaudet. Antigen processing and presentation: TAPping into ABC transporters. Curr Opin Immunol. (2009) 21: 84-91. Epub 2009 Mar 2. PubMed: 19261456

E. Procko, M.L. O'Mara, W.F. Bennett, D.P. Tieleman and R. Gaudet. The mechanism of ABC transporters: general lessons from structural and functional studies of an antigenic peptide transporter. FASEB Journal. (2009) 23: 1287-302. Epub 2009 Jan 27. PubMed: 19174475

R. Gaudet. The ABCs of trans(porter) inhibition. Nat Chem Biol. (2008) 4: 454-5. PubMed:18641624

E. Procko and R. Gaudet. Functionally important interactions between the nucleotide-binding domains of an antigenic peptide transporter. Biochemistry. (2008) 47: 5699-708. Epub 2008 May 2. PubMed: 18452308

E. Procko, I. Ferrin-O'Connell, S-.L. Ng and R. Gaudet. Distinct structural and functional properties of the ATPase sites in an asymmetric ABC transporter. Mol Cell. (2006) 24: 51-62. PubMed 2IXE 2IXF 2IXG

E. Procko, G. Raghuraman, D.C. Wiley, M. Raghavan and R. Gaudet. Identification of domain boundaries within the N-termini of TAP1 and TAP2 and their importance in tapasin binding and tapasin-mediated increase in peptide loading of MHC class I. Immunol. Cell. Biol. (2005) 83: 475-482. PubMed

S. Misaghi, Z.-Y. J. Sun, P. Stern, R. Gaudet, G. Wagner and H. Ploegh. A structural and functional analysis of the human cytomegalovirus US3 protein. J. Virol. (2004) 78: 413-423. PubMed

R. Gaudet and D.C. Wiley. Structure of the ABC ATPase domain of human TAP1, the transporter associated with antigen processing. EMBO J. (2001) 20: 4964-4972. PubMed 1JJ7

B.E. Gewurz, R. Gaudet, D. Tortorella, E.W. Wang and H.L. Ploegh. Virus subversion of immunity: a structural perspective. Curr. Opin. Immunol. (2001) 13: 442-450. PubMed

B.E. Gewurz, R. Gaudet, D. Tortorella, E.W. Wang, H.L. Ploegh and D.C. Wiley. Antigen presentation subverted: Structure of the human cytomegalovirus protein US2 bound to the class I molecule HLA-A2. Proc. Natl Acad. Sci. USA (2001) 98: 6794-6799. PubMed 1IM3

 

Cadherins Superfamily Neuronal Development of Hearing and Deafness

R.E. Powers, R. Gaudet*, and M. Sotomayor* A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15. Structure (2017) 25:482-495. PubMed: 28238533 PMCID: PMC5363953 PDB: 5T4M 5T4N

J. M. Nicoludis, B. E. Vogt, A. G. Green, C. P. Schärfe, D. S. Marks, and R. Gaudet Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4. Elife. (2016) 5. pii, e18449. PubMed: 27472898 PMCID: PMC5001838 PDB: 5K8R

J. M. Nicoludis, S.-Y. Lau, C. P. I. Schärfe, D. S. Marks, W. A. Weihofen, and R. Gaudet Structure and Sequence Analyses of Clustered Protocadherins Reveal Antiparallel Interactions that mediate homophilic specificity. Structure (2015) 23, 2087-2096. PubMed: 26481813 PMCID: PMC4635037 PDB: 4Z18 4Z19

M. Sotomayor, R. Gaudet, and D. P. Corey (2014) Sorting out a promiscuous superfamily: towards cadherin connectomics. Trends Cell Biol. 24, 524-36. PubMed: 24794279 PMCID: PMC4294768

R. Geng, M. Sotomayor, K. J. Kinder, S. R. Gopal, J. Gerka-Stuyt, D. H. Chen, R. E. Hardisty-Hughes, G. Ball, A. Parker, R. Gaudet, D. Furness, S. D. Brown, D. P. Corey, and K. N. Alagramam. Noddy, a mouse harboring a missense mutation in protocadherin-15, reveals the impact of disrupting a critical interaction site between tip-link cadherins in inner ear hair cells. Journal of Neuroscience (2013) 33, 4395-4404. PubMed: 23467356 PMCID: PMC3618968

M. Sotomayor, W.A. Weihofen, R. Gaudet and D.P. Corey. Structure of a force-conveying cadherin bond essential for inner-ear mechanotransduction. Nature (2012) Published online 2012-11-07. doi:10.1038/nature11590 PubMed: 23135401 PMCID: PMC3518760 4APX 4AXW 4AQ8 4AQA 4AQE

M. Sotomayor, W.A. Weihofen, R. Gaudet and D.P. Corey. Structural Determinants of Cadherin-23 Function in Hearing and Deafness. Neuron (2010) 66: 85-100. PubMed: 20399731 PMCID: PMC2948466 2WBX 2WCP 2WHV 2WD0

 

Ubiquitylation Pathways

K. Artavanis-Tsakonas, W.A. Weihofen, J.M. Antos, B.I. Coleman, C.A. Comeaux, M.T. Duraisingh, R. Gaudet and H.L. Ploegh
Characterization and structural studies of the Plasmodium falciparum ubiquitin and NEDD8 hydrolase UCHL3.
J. Biol. Chem. (2010) 285: 6857-66.
PubMed 2WDT 2WE6

C. Schlieker, W.A. Weihofen, E. Frijns, L.M. Kattenhorn, R. Gaudet and H.L. Ploegh
Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes.
Mol Cell. (2007) 25: 677-87.
PubMed 2J7Q

S. Misaghi, P.J. Galardy, W.J. N. Meester, H. Ovaa, H.L. Ploegh and R. Gaudet
Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate.
J. Biol. Chem. (2005) 280: 1512-1520.
PubMed 1XD3

 

Phosducin Regulation of G Protein Signaling

M. Obin, B.Y. Lee, C. Thulin, G. Meinke, A. Bohm, R.H. Lee, R. Gaudet, J.A. Hopp, V.Y. Arshavsky, B.M. Willardson and A. Taylor
Ubiquitylation of Transducin (T) subunits: Regulation by Phosducin.
J. Biol. Chem. (2002) 277: 44566-44575.
PubMed: 12215439 

R. Gaudet, J.R. Savage, J.N. McLaughlin, B.M. Willardson and P.B. Sigler
A molecular mechanism for the phosphorylation-dependent regulation of heterotrimeric G Proteins by phosducin.
Mol. Cell (1999) 3: 649-660.
PubMed: 10360181 1B9X 1B9Y

A. Bohm, R. Gaudet and P.B. Sigler
Structural aspects of heterotrimeric G-protein signaling.
Curr. Opin. Biotechnol. (1997) 8: 480-487.
PubMed: 9265729

R. Gaudet, A. Bohm and P.B. Sigler
Crystal structure at 2.4 Å resolution of the complex of transducin bg and its regulator, phosducin.
Cell (1996) 87: 577-588.
PubMed: 8898209 2TRC

 

Additional Works

J.M. Nicoludis and R. Gaudet (2017) Applications of sequence coevolution in membrane protein biochemistry. Biochim Biophys Acta Oct 7. pii: S0005-2736(17)30314-0. PubMed: 28993150 PMCID: in process

Bradshaw N, Levdikov VM, Zimanyi CM, Gaudet R, Wilkinson AJ, Losick R. A widespread family of serine/threonine protein phosphatases shares a common regulatory switch with proteasomal proteases. Elife. 2017;6. PubMed: 28527238 PMCID: PMC5468089 PDB: 5MQH 5UCG 

P.A. Meyer, S. Socias, J. Key, E. Ransey, E.C. Tjon, A. Buschiazzo, M. Lei, C. Botka, J. Withrow, D. Neau, K. Rajashankar, K. S. Anderson, R. H. Baxter, S. C. Blacklow, T. J. Boggon, A. M. Bonvin, D. Borek, T. J. Brett, A. Caflisch, C. I. Chang, W. J. Chazin, K. D. Corbett, M. S. Cosgrove, S. Crosson, S. Dhe-Paganon, E. Di Cera, C. L. Drennan, M. J. Eck, B. F. Eichman, Q. R. Fan, A. R. Ferré-D'Amaré, J. C. Fromme, K. C. Garcia, R. Gaudet, P. Gong, S. C. Harrison, E. E. Heldwein, Z. Jia, R. J. Keenan, A. C. Kruse, M. Kvansakul, J.S. McLellan, Y. Modis, Y. Nam, Z. Otwinowski, E. F. Pai, P. J. Pereira, C. Petosa, C. S. Raman, T. A. Rapoport, A. Roll-Mecak, M. K. Rosen, G. Rudenko, J. Schlessinger, T. U. Schwartz, Y. Shamoo, H. Sondermann, Y. J. Tao, N. H. Tolia, O. V. Tsodikov, K. D. Westover, H. Wu, I. Foster, J. S. Fraser, F. R. Maia, T. Gonen, T. Kirchhausen, K. Diederichs, M. Crosas, P. Sliz Data publication with the structural biology data grid supports live analysis. Nature Communications (2016) 7, 10882. PubMed: 26947396 PMCID: PMC4786681

T. A. Sysoeva, L.B. Bane, D. Y. Xiao, B. Bose, S. S. Chilton, R. Gaudet, B. M. Burton Structural characterization of the late competence protein ComFB from Bacillus subtilis. Biosci Rep. (2015) 35, e00183. PubMed: 25423369 PMCID: PMC4381287 PDB: 4WAI

P.C. Sabeti, P. Varilly, B. Fry. J. Lohmueller, E. Hostetter, C. Costapas, X. Xie, E.H. Byrne, S.A. McCarroll, R. Gaudet, S.F. Schaffner, E.S. Lander & The International HapMap Consortium. Genome-wide detection and characterization of positive selection in human populations. Nature (2007) 449, 913-918. PubMed: 17943131 PMCID: PMC2687721 

M. Holm, C.S. Hardtke, R. Gaudet and X.-W. Deng Identification and characterization of a structural motif that confers specific interaction with the WD40 repeat domain of Arabidopsis COP1. EMBO J. (2001) 20: 118-127. PubMed: 11226162 PMCID: PMC140188