Andreane Cartier Research Fellow, Vascular Biology Program, Boston Children's HospitalResearch Fellow, Harvard Medical School I did my PhD in the lab of Dr Jean-Luc Parent in Sherbrooke, Quebec. I characterized a newly identified interacting protein (WDR36) of the beta isoform of the thromboxane A2 receptor (TPβ), a GPCR that regulates platelets aggregation and vascular smooth muscle cells constriction. My training with Dr Timothy Hla gives me the opportunity to apply to mouse models my knowledge of GPCR signaling, molecular biology and transcriptional gene regulation. Project 1. Role of endothelial cell S1P1 and S1P2 receptors in tumor angiogenesis and tumor growth. Project 2. Whole genome siRNA screen to identify new targets of the endocytic pathway of S1P1 induced by Fingolimod, a synthetic functional antagonist of S1P1 and FDA approved molecule, currently used as a treatment for multiple sclerosis. Project 3. Role of S1P1 and S1P2 in vascular dysfunction caused by a chronic inflammatory environment in vitro and in vivo, and how the imbalance of these two receptors leads to endothelial dysfunction and vascular injury, key processes in cardiovascular diseases development. Publications Highlights Binda C, Génier S, Cartier A, Larrivée JF, Stankova J, Young JC and Parent JL. A G protein-coupled receptor and the intracellular synthase of its agonist functionally cooperate. J Cell Biol. 2014 Feb 3;204(3):377-93. Lachance V, Cartier A, Génier S, Munger S, Germain P, Labrecque P and Parent JL. Regulation of b2-adrenergic receptor maturation and anterograde trafficking by an interaction with Rab geranylgeranyltransferase: modulation of Rab geranylgeranylation by the receptor. J Biol Chem. 2011 Nov 25;286(47):40802-13. Cartier A., Parent A., Labrecque P, Laroche G and Parent JL. WDR36 acts as a scaffold protein tethering a G protein-coupled receptor, Gaq and a phospholipase Cb in a signaling complex. J Cell Sci. 2011 Oct 1;124(Pt 19):3292-304. Email: Andreane.Cartier@childrens.harvard.edu Phone: (617) 919-2404
Eric Engelbrecht Research Assistant, Vascular Biology Program, Boston Children's Hospital I joined the Hla lab in 2014 after graduating from Kenyon College with a B.A. in Molecular Biology. I am interested in determining the epigenomic landscape of aortic endothelial cells from regions of laminar and disturbed flow to discover specific factors that regulate heterogenous phenotypes within a single cell-type. Publication Highlights Blaho V. A., Galvani S., Engelbrecht E., et al. HDL-bound sphingosine-1-phosphate restrains lymphopoiesis and neuroinflammation. Nature. 2015;523(7560):342–346. doi: 10.1038/nature14462 Scott H. Freeburg, Eric Engelbrecht, and Wade H. Powell. Subfunctionalization of paralogous aryl hydrocarbon receptors from the frog Xenopus laevis: Distinct target genes and differential responses to specific agonists in a single cell type. Toxicological Sciences. 2017; 155 (2): 337-347. doi: 10.1093/toxsci/kfw212 Email: Eric.Engelbrecht@childrens.harvard.edu Phone: (617) 919-2167
Weijing Feng Graduate Student, Vascular Biology Program, Boston Children's Hospital Graduate Student, Harvard Medical School I received my Bachelor Degree in clinical medicine from Guangzhou Medical University. Further, I got my Medical Degree in cardiovascular medicine from Sun Yat-sen University. I did my PhD-training in Dr. Minsheng Chen’s lab at Southern Medical University. I joined Dr. Tim Hla’s lab in October, 2019 to continue my PhD training as a joint training PhD student. I am currently investigating the role of G proteins and G-protein-coupled receptors (GPCRs) in the development of cardiovascular diseases. I am interested in determining protein-protein interactions with NanoBiT assay system to discover specific factors that regulate vascular cells function. Publication Highlights Zhang K, Zhang Y, Feng W, Chen R, Chen J, Touyz RM, Wang J, Huang H. Interleukin-18 Enhances Vascular Calcification and Osteogenic Differentiation of Vascular Smooth Muscle Cells Through TRPM7 Activation. Arterioscler Thromb Vasc Biol. (2017) Feng W, Zhang K, Liu Y, Chen J, Cai Q, He W, Zhang Y, Wang M, Wang J, Huang H. Advanced oxidation protein products aggravate cardiac remodeling via cardiomyocyte apoptosis in chronic kidney disease. Am J Physiol Heart Circ Physiol. (2018) Email: weijing.feng@childrens.harvard.edu
Bongnam Jung Research Associate, Vascular Biology Program, Boston Children's HospitalResearch Associate, Harvard Medical School Bongnam did her PhD in Cellular and Developmental Biology at Cornell University, New York. During her study, she worked on the role of Sphingosine 1-phosphate (S1P) receptor 1 in retinal vascular development using in vitro and in vivo systems under the guidance of Dr. Timothy Hla. She went to Sweden to do her Post-doctoral training with Dr. Christer Betsholtz to learn more about blood vessels. She was interested in characterizing pericytes, the cells surrounding the endothelium, over the course of brain development in mouse. She also investigated the involvement of fibroblast growth factor binding protein 1 (FGFBP1) in pericyte deficiency-mediated blood brain barrier breakdown. She joined back the Hla lab in Feb, 2019 and wants to explore the function of S1P receptors in the central nervous system and their underlying mechanisms. Publication Highlights 1. Jung B, Arnold TD, Raschperger E, Gaengel K, Betsholtz C.. Visualization of vascular mural cells in developing brain using genetically labeled transgenic reporter mice. J Cereb Blood Flow Metab. (2017) 2. Ding BS, Liu CH, Sun Y, Chen Y, Swendeman SL, Jung B, Chavez D, Cao Z, Christoffersen C, Nielsen LB, Schwab SR, Rafii S, Hla. HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P1) promotes regeneration and suppresses fibrosis in the liver. JCI Insight. (2016) 3. He L, Vanlandewijck M, Raschperger E, Andaloussi Mäe M, Jung B, Lebouvier T, Ando K, Hofmann J, Keller A, Betsholtz C. Analysis of the brain mural cell transcriptome. Sci Rep. (2016) Email: Bongnam.Jung@childrens.harvard.edu Phone: 617-919-2166
Andrew Kuo Research Fellow, Boston Children's HospitalResearch Fellow, Harvard Medical School Andrew received his Bachelor Degree in Zoology from National Taiwan University. Further, he completed his PhD training in Cell Biology under the laboratory of Dr. William Sessa, where he became interested in lipid metabolism in the vasculature system, particular triglyceride metabolism in the endothelium. His thesis project characterized Lipid Droplets (LD) for the first time in endothelial cells and demonstrated endothelium LD function as a buffer for lipid homeostasis and transport lipids to subendothelial layer. Andrew joined the Hla lab in May, 2017 to continue his postdoctoral training studying sphingolipid biology. His current research interests include understanding S1P compartmentalization and availability in the blood brain barrier and investigating the functions of S1P bound to different chaperones in vitro and in vivo. Publications Highlights Kuo, A., Lee, M. Y., Sessa, W. C.. Lipid Droplet Biogenesis and Function in the Endothelium. Circ Res. (2017) Jozsef, L., Tashiro, K., Kuo, A., Park, E., Skoura, A., Albinsson, S., Rivera-Molina, F., Harrison, K. D., Iwakiri Y, Toomre D, Sessa WC. Reticulon 4 is necessary for endoplasmic reticulum tubulation, STIM1-Orai1 coupling, and store-operated calcium entry. J Biol. Chem. (2014) Harrison, K. D., Park, E., Gao, N., Kuo, A., Rush, J. S., Waechter, C. J., Lehrman, M. A. and Sessa, W. C.. NgBR is essential for cellular dolichol synthesis and protein glycosylation. EMBO J. (2011). Email: Andrew.Kuo@childrens.harvard.edu Phone: (617) 919-2403
Michel Levesque Research Fellow, Vascular Biology Program, Boston Children's HospitalResearch Fellow, Harvard Medical School The focus of my research as a graduate student was the application of a molecular tool for gene targeting, previously engineered in the laboratory of my PhD mentor, Dr. Jean-Pierre Perreault. More precisely, I have used highly specific ribozymes to block gene expression and the replication of two different RNA viruses (HCV and HIV). I have also developed an efficient design process to select active ribozymes against genes of interest. Through this work, I have gained experience on working with RNA in different contexts and in the characterization of gene expression in mammalian cells using techniques such as RNAse protection assays, Northern blots and luciferase assays. I have also developed an expertise in gene targeting and nucleic acid based technology design. After I received my PhD, I moved to MSKCC to study, as research fellow, the roles of alternative polyadenylation of genes related to cancer (CD47 and PTEN). I have used protein (GFP fusion and immunofluorescence) and mRNA (FISH) localisation to assess the role of short and long 3’UTR isoforms. I have also developed western blot and immunoprecipitation conditions to evaluate the isoforms expression and post-translational modifications of a key element of the polyadenylation complex (FIP1L1). Then I moved to Weill Cornell Medicine to work with Dr. Timothy Hla. My research has been focusing on developing a method to study gene expression profile in mouse aortic endothelial cells. Specifically, I have been trying to use the S1PR1 GFP signaling mice model to collect separately endothelial cells exposed to laminar shear stress and disturbed blood flow to characterize their transcriptome. I have also started a project focusing on the transcriptional regulation of S1PR1. Publication Highlights Lévesque MV, Lévesque D, Brière FP, Perreault J-P. (2010) Investigating a New Generation of Ribozymes in Order to Target HCV. PLoS One 5: e9627. PMID: 20224783 Lévesque MV, Perreault J-P. (2012) Target Induced SOFA-HDV Ribozyme. Methods Mol Biol. 848:369-84 PMID: 22315081 Scarborough RJ, Lévesque MV, Boudrias-Dalle E, Chute IC, Daniels SM, Ouellette RJ, Perreault JP, and Gatignol A. (2014) A conserved target site in HIV-1 Gag RNA is accessible to both HDV Ribozyme and short hairpin RNA activities. Mol Ther Nucleic Acids. 3:e178 PMID: 25072692 Email: Michel.Levesque@childrens.harvard.edu Phone: (617) 919-2167
Daniel Lin Research Fellow, Vascular Biology Program, Boston Children's HospitalResearch Fellow, Harvard Medical School Daniel did his Ph.D. training by joining the double Ph.D. program at both University of Tsukuba, Japan and National Taiwan University, Taiwan. He received the first Ph.D. at University of Tsukuba under Dr. Yasunori Kanaho, where he characterized the lymphangiogenesis role of small GTPase Arf6 in embryonic development and cancer progression and how Arf6 regulates intracellular trafficking of b1 integrin (Lin et al., Scientific Report, 2017). In addition, he received the second Ph.D. from National Taiwan University under Dr. Hsinyu Lee, where he found the VEGF-C signaling pathway mediated by lipid mediator lysophosphatidic acid (LPA) in human prostate cancer, and how it regulates tumor lymphangiogenesis and lymphatic metastasis (Lin et al., BBA-Moll Cell Biol Lipids, 2018). Daniel joined the Hla lab in September 2019 to investigate the role of S1P receptor 2 in lymphatic vessel development at embryonic stages and pathological conditions. Publication Highlights Lin YC, Chen CC, Chen WM, Lu KY, Shen TL, Jou YC, Shen CH, Ohbayashi N, Kanaho Y, Huang YL, Lee H. (2018). LPA1/3 Signaling Mediates Tumor Lymphangiogenesis through Promoting CRT Expression in Prostate Cancer. Biochimica et Biophysica Acta – Molecular and Cell Biology of Lipids. Huang YL, Lin YC, Lin CC, Chen WM, Chen B, Lee H. (2018). High Glucose Induces VEGF-C Expression via the LPA1/3-Akt-ROS-LEDGF Signaling Axis in Human Prostate Cancer PC-3 Cells. Cellular Physiology and Biochemistry. Lin YC, Ohbayashi N, Hongu T, Katagiri N, Funakoshi Y, Lee H, Kanaho Y. (2017). Arf6 in Lymphatic Endothelial Cells Regulates Lymphangiogenesis by Controlling Directional Cell Migration. Scientific Reports. Email: Daniel.Lin@childrens.harvard.edu
Colin Niaudet Research Associate, Vascular Biology Program, Boston Children's Hospital During his PhD, Colin studied the protective role of sphingosine-1-phosphate upon radiation of the endothelium, under the direction of Francois PARIS (INSERM, Nantes, France). He then joined Pr. Christer BETSHOLTZ’s lab, where he helped understanding S1PR1 function in the developing vascular system (Karolinska institute, Stockholm, Sweden). He also characterized the physiological roles of another vascular GPCR, the adhesion receptor Gpr116, especially in the endothelium of the central nervous system (Uppsala university, Sweden). He joined Pr. Timothy HLA’s lab in 2019, and continue to characterize the role of S1P signaling, focusing on vascular maintenance in the retina, while pursuing its study of Gpr116 functions. Publication highlights: Niaudet C, Petkova M, Jung B, Lu S, Laviña B, Offermanns S, Brakebusch C, Betsholtz C. Adgrf5 contributes to patterning of the endothelial deep layer in retina. Angiogenesis.(2019) Gaengel K, Niaudet C, Hagikura K, Laviña B, Muhl L, Hofmann JJ, Ebarasi L, Nyström S, Rymo S, Chen LL, Pang MF, Jin Y, Raschperger E, Roswall P, Schulte D, Benedito R, Larsson J, Hellström M, Fuxe J, Uhlén P, Adams R, Jakobsson L, Majumdar A, Vestweber D, Uv A, Betsholtz C. The sphingosine-1-phosphate receptor S1PR1 restricts sprouting angiogenesis by regulating the interplay betweenVE-cadherin and VEGFR2. Dev Cell. (2012) Bonnaud S, Niaudet C, Legoux F, Corre I, Delpon G, Saulquin X, Fuks Z, Gaugler MH, Kolesnick R, Paris F. Sphingosine-1-phosphate activates the AKT pathway to protect small intestines from radiation-induced endothelial apoptosis. Cancer Res. (2010 Colin.Niaudet@childrens.harvard.edu
Takahiro Seno Research Fellow, Vascular Biology Program, Boston Children's HospitalResearch Fellow, Harvard Medical School I am a clinical immunologist, specializing in rheumatology, allergology and respiratory medicine. I worked as a specialist physician and assistant professor at Kyoto Prefectural University of Medicine, Japan. I did my PhD-training in Dr. Yutaka Kawahito's lab (Department of Inflammation and Immunology) at Kyoto Prefectural University of Medicine, Japan. I was trained in vascular inflammation and lipid metabolism with rheumatic diseases. After receiving my medical doctoral degree, I engaged in research about vascular inflammation, interstitial pneumonitis and development of peptide biomarkers of rheumatoid arthritis. I joined Dr. Tim Hla’s lab in July 2017. In this lab, I investigate the role of S1PR1 and S1PR4 in polymorphonuclear (PMN) cells NETosis. NETs is involved in many diseases, such as systemic lupus erythematous, rheumatoid arthritis, ANCA-associated vasculitis, atherosclerosis, cancer and diabetes mellitus, that have so many clinical unmet needs. Our findings may contribute to resolve the unmet needs. And I also explore the role of ApoM-Fc as a potential therapeutic in anaphylaxis. This findings reveal how S1PRs regulate circulatory collapse, and may be new therapeutic target for the broad range of clinical settings with circulatory collapse. Email: Takahiro.Seno@childrens.harvard.edu
Steven Swendeman Research Associate, Vascular Biology Program, Boston Children's HospitalResearch Associate, Harvard Medical School I am currently investigating the role of the HDL-associated lipid-chaperone, Apolipoprotein M, in vascular function and physiology in normal homeostasis and in human diseases. I did my PhD in Immunology at the Tufts University School of Medicine and Post-doctoral fellowships in Human Genetics at the Whitehead Institute at MIT and in Oncology at the Memorial Sloan-Kettering Cancer Center. I did further research in Vascular Biology at Weill-Cornell Medical College and The Hospital for Special Surgery. Publication Highlights Ding BS, Liu CH, Sun Y, Chen Y, Swendeman SL, Jung B, Chavez D, Cao Z, Christoffersen C, Nielsen LB, Schwab SR, Rafii S, Hla T HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P1) promotes regeneration and suppresses fibrosis in the liver. JCI Insight. 2016 Dec 22;1(21):e87058. doi: 10.1172/jci.insight.87058. Christensen PM, Liu CH, Swendeman SL, Obinata H, Qvortrup K, Nielsen LB, Hla T, Di Lorenzo A, Christoffersen C. Impaired endothelial barrier function in apolipoprotein M-deficient mice is dependent on sphingosine-1-phosphate receptor 1. FASEB J. 2016 Jun;30(6):2351-9. doi: 10.1096/fj.201500064. Epub 2016 Mar 8. Galvani S, Sanson M, Blaho VA, Swendeman SL, Obinata H, Conger H, Dahlbäck B, Kono M, Proia RL, Smith JD, Hla T. HDL-bound sphingosine 1-phosphate acts as a biased agonist for the endothelial cell receptor S1P1 to limit vascular inflammation. Sci Signal. 2015 Aug 11;8(389):ra79. doi: 10.1126/scisignal.aaa2581. Email: Steven.Swendeman@childrens.harvard.edu Phone: (617) 919-2166