@article {112251,
	title = {Transposase promotes double strand breaks and single strand joints at Tn10 termini in vivo.},
	journal = {Cell},
	volume = {39},
	number = {1},
	year = {1984},
	month = {1984 Nov},
	pages = {181-90},
	abstract = {We present evidence that Tn10 transposase promotes double strand breaks and single strand joints at Tn10 termini in vivo. Plasmids containing a shortened Tn10 element and a transposase overproducer fusion give rise, upon transposase induction, to new DNA species. The most prominent class is a circularized transposon molecule whose structure suggests that it arises from double strand breakage at the two transposon ends followed by covalent joining between the 3{\textquoteright} and 5{\textquoteright} ends of one of the two strands. We have used formation of the circularized transposon as a physical assay for the interaction between transposase and different mutant and wild-type termini. These experiments show that transposase protein interacts preferentially with the genetically most active termini in a way that suppresses productive interaction with weaker termini present on the same substrate molecule.},
	keywords = {Base Sequence, Cloning, Molecular, DNA Restriction Enzymes, DNA Transposable Elements, Escherichia coli, Nucleic Acid Hybridization, Nucleotidyltransferases, Plasmids, Transposases},
	issn = {0092-8674},
	author = {Morisato, D and Kleckner, N}
}
