Electron microscopy is an ideal imaging technique to study biological cells at the nanoscale. However, typically electron microscopy only provides static black-and-white images. In an effort to enable multicolor and time-resolved electron microscopy, my laboratory will develop dedicated molecular probes and instrumentation for new correlative light and electron microscopy modalities, and build next-generation biophysical tools for time-resolved cryo-vitrification and high-resolution imaging of cells. These methods will be used to elucidate the nanoscale protein and membrane dynamics of G protein-coupled receptor (GPCR) signaling in differentiated cells and complex tissues.
September 5, 2019