@article {1634923,
	title = {Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer},
	journal = {Cell Rep},
	volume = {25},
	number = {12},
	year = {2018},
	month = {2018 Dec 18},
	pages = {3504-3518.e6},
	abstract = {The exact identity of castrate-resistant (CR) cells and their relation to CR prostate cancer (CRPC) is~unresolved. We use single-cell gene profiling to~analyze the molecular heterogeneity in basal and luminal compartments. Within the luminal compartment, we identify a subset of cells intrinsically resistant to castration with a bi-lineage gene expression pattern. We discover LY6D as a marker of CR prostate progenitors with multipotent differentiation and enriched organoid-forming capacity. Lineage tracing further reveals that LY6D+ CR luminal cells can produce LY6D- luminal cells. In contrast, in luminal cells lacking PTEN, LY6D+ cells predominantly give rise to LY6D+ tumor cells, contributing to high-grade PIN lesions. Gene expression analyses in patients{\textquoteright} biopsies indicate that LY6D expression correlates with early disease progression, including progression to CRPC. Our studies thus identify a subpopulation of luminal progenitors characterized by LY6D expression and intrinsic castration resistance. LY6D may serve as a prognostic maker for advanced prostate cancer.},
	keywords = {Animals, Biomarkers, Tumor, Carcinogenesis, Cell Adhesion Molecules, Cell Lineage, Disease Progression, Epithelial Cells, GPI-Linked Proteins, Humans, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neoplastic Stem Cells, Organoids, Prostate, Prostatic Neoplasms, Castration-Resistant, Regeneration, Single-Cell Analysis},
	issn = {2211-1247},
	doi = {10.1016/j.celrep.2018.11.069},
	author = {Barros-Silva, Jo{\~a}o D and Linn, Douglas E and Steiner, Ivana and Guo, Guoji and Ali, Adnan and Pakula, Hubert and Ashton, Garry and Peset, Isabel and Brown, Michael and Clarke, Noel W and Bronson, Roderick T. and Yuan, Guo-Cheng and Orkin, Stuart H and Li, Zhe and Baena, Esther}
}
