@article {1634935,
	title = {Lineage tracing of mammary epithelial cells using cell-type-specific cre-expressing adenoviruses},
	journal = {Stem Cell Reports},
	volume = {2},
	number = {6},
	year = {2014},
	month = {2014 Jun 03},
	pages = {770-9},
	abstract = {Lineage tracing using Cre/lox transgenic mice provides a powerful tool for studying normal mammary epithelial cell (MEC) development and the cellular origins of mammary tumors under physiological settings. However, generation of new transgenic mice for lineage-tracing purposes is often time consuming. Here, we report a lineage-tracing tool for MECs based on intraductal injection of lineage-specific Cre-expressing adenovirus (Ad-Cre). Using well-characterized promoters for Keratin 8 and Keratin 14, we generated lineage-specific Ad-Cre lines for luminal and basal MECs, respectively. By pulse-chase lineage tracing using these Ad-Cre lines, we showed that luminal and basal lineages are largely self-sustained and that IRS1 and IRS2 are essential for maintaining the basal lineage; we also showed that heterogeneous mammary tumors can be induced from luminal MECs in mice carrying the Etv6-NTRK3 fusion gene. Overall, we validated the Ad-Cre system as a promising and efficient tool for fate mapping of normal and malignant cells in adult tissues.},
	keywords = {Adenoviridae, Animals, Epithelial Cells, Female, Insulin Receptor Substrate Proteins, Keratin-14, Keratin-8, Mammary Glands, Animal, Mice, Mice, Transgenic, Oncogene Proteins, Fusion},
	issn = {2213-6711},
	doi = {10.1016/j.stemcr.2014.04.004},
	author = {Tao, Luwei and van Bragt, Maaike P A and Laudadio, Elizabeth and Li, Zhe}
}
