@article {1635520,
	title = {T-lymphoid, megakaryocyte, and granulocyte development are sensitive to decreases in CBFbeta dosage},
	journal = {Blood},
	volume = {109},
	number = {1},
	year = {2007},
	month = {2007 Jan 01},
	pages = {11-21},
	abstract = {The family of core-binding factors includes the DNA-binding subunits Runx1-3 and their common non-DNA-binding partner CBFbeta. We examined the collective role of core-binding factors in hematopoiesis with a hypomorphic Cbfb allelic series. Reducing CBFbeta levels by 3- or 6-fold caused abnormalities in bone development, megakaryocytes, granulocytes, and T cells. T-cell development was very sensitive to an incremental reduction of CBFbeta levels: mature thymocytes were decreased in number upon a 3-fold reduction in CBFbeta levels, and were virtually absent when CBFbeta levels were 6-fold lower. Partially penetrant consecutive differentiation blocks were found among early T-lineage progenitors within the CD4- CD8- double-negative 1 and downstream double-negative 2 thymocyte subsets. Our data define a critical CBFbeta threshold for normal T-cell development, and situate an essential role for core-binding factors during the earliest stages of T-cell development.},
	keywords = {Alleles, Animals, Bone Development, Cell Lineage, Colony-Forming Units Assay, Core Binding Factor alpha Subunits, Core Binding Factor beta Subunit, Granulocytes, Hematopoiesis, Liver, Megakaryocytes, Mice, Mice, Inbred C57BL, Mice, Knockout, Phenotype, Radiation Chimera, Spleen, T-Lymphocyte Subsets, Thymus Gland},
	issn = {0006-4971},
	doi = {10.1182/blood-2006-05-021188},
	author = {Talebian, Laleh and Li, Zhe and Guo, Yalin and Gaudet, Justin and Speck, Maren E and Sugiyama, Daisuke and Kaur, Prabhjot and Pear, Warren S and Maillard, Ivan and Speck, Nancy A}
}
