T-lymphoid, megakaryocyte, and granulocyte development are sensitive to decreases in CBFbeta dosage

Citation:

Laleh Talebian, Zhe Li, Yalin Guo, Justin Gaudet, Maren E Speck, Daisuke Sugiyama, Prabhjot Kaur, Warren S Pear, Ivan Maillard, and Nancy A Speck. 2007. “T-lymphoid, megakaryocyte, and granulocyte development are sensitive to decreases in CBFbeta dosage.” Blood, 109, 1, Pp. 11-21.

Abstract:

The family of core-binding factors includes the DNA-binding subunits Runx1-3 and their common non-DNA-binding partner CBFbeta. We examined the collective role of core-binding factors in hematopoiesis with a hypomorphic Cbfb allelic series. Reducing CBFbeta levels by 3- or 6-fold caused abnormalities in bone development, megakaryocytes, granulocytes, and T cells. T-cell development was very sensitive to an incremental reduction of CBFbeta levels: mature thymocytes were decreased in number upon a 3-fold reduction in CBFbeta levels, and were virtually absent when CBFbeta levels were 6-fold lower. Partially penetrant consecutive differentiation blocks were found among early T-lineage progenitors within the CD4- CD8- double-negative 1 and downstream double-negative 2 thymocyte subsets. Our data define a critical CBFbeta threshold for normal T-cell development, and situate an essential role for core-binding factors during the earliest stages of T-cell development.
Last updated on 02/07/2023